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. 2020 Oct 22;22:1092–1106. doi: 10.1016/j.omtn.2020.10.021

Figure 1.

Figure 1

Identification of Notochordal Cells (NCs)

(A) Schematic diagram for isolation of NCs and chondrocyte-like nucleus pulposus cells (NPs). (B) Isolated NCs and NPs. Bar, 50 μm. (C) Immunofluorescence staining of cytokeratin 8 (CK8) and vimentin expression in NCs and NPs. Bar, 50 μm. (D) Western blotting analysis of CK8 and vimentin expression in NCs and NPs. (E) RT-PCR analysis of brachyury expression in NCs and NPs. The expression of brachyury in NPs was normalized to 1. Mean ± SEM is provided (n = 3). ∗∗p < 0.01.