Figure 6.

RNA-Seq-Identified NF-κB Pathway Is Associated with miR-10a in OA-FLSs

(A) KEGG signaling pathway enrichment analysis identified the most relevant pathways of miR-10a in OA-FLSs. (B) miR-10a repressed NF-κB reporter activity in OA-FLSs. The stars indicated the significant differences from control (n = 3). (C) miR-10a repressed nucleus (NE) p65 in OA-FLSs (n = 3). (D) The effects of blockage of the NF-κB pathway on proliferation of OA-FLSs (n = 3). (E) The effects of blockage of the NF-κB pathway on migration of OA-FLSs (n = 3). (F) The effects of inhibition of the NF-κB pathway on the secretion of pro-inflammatory cytokines in OA-FLS (n = 3). (G) The effects of blockage of NF-κB pathway mRNA expressions of fibrotic markers in OA-FLSs (n = 3). Data represent means ± SEM; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, as determined by the Student’s t test.