Figure 7.

TWIST1 Is an Upstream Regulator of miR-10a in OA-FLSs

(A) The expression of TWIST1 in synovium from OA patients (n = 12) and controls (n = 5). (B and C) The expression of miR-10a in TWIST1 KD (B) or OE (C) OA-FLSs (n = 3). (D) The correlation (Pearson’s correlation coefficient) of TWIST1 and miR-10a in OA synovium (n = 25). (E) Luciferase reporter assays showed that TWIST1 directly binds to the E box in the promoter region of miR-10a, a Renilla luciferase-encoding internal control plasmid for normalization (n = 3). (F) ChIP assays demonstrated the binding of TWIST1 to miR-10a promoter that contains an E box. Data represent means ± SEM; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, as determined by the Student’s t test.