Figure 2.

SMSC-EV-Packaged miR-31 Induces Cartilage Formation by Targeting KDM2A In Vitro

(A) The expression of miR-31 determined by qRT-PCR in chondrocytes transfected with miR-31 mimic. (B) The expression of KDM2A determined by qRT-PCR in chondrocytes transfected with miR-31 mimic. ∗p < 0.05 compared with chondrocytes transfected with mimic-NC. (C) The expression of miR-31 determined by qRT-PCR in chondrocytes treated with EVs from miR-31 inhibitor-transfected SMSCs. (D) The expression of KDM2A determined by qRT-PCR in chondrocytes treated with EVs from the miR-31 inhibitor transfected with SMSCs. (E) Chondrocyte proliferation measured by the CCK-8 assay in response to EVs from miR-31 inhibitor-transfected SMSCs. (F) The number of migrated chondrocytes measured by the Transwell assay in response to EVs from miR-31 inhibitor-transfected SMSCs. (G) Representative images of migrating chondrocytes in response to EVs from miR-31 inhibitor-transfected SMSCs (×200). ∗p < 0.05 compared with the control chondrocytes without any treatment; #p < 0.05 compared with chondrocytes treated with EVs from SMSCs transfected with inhibitor-NC. Data are shown as mean ± standard deviation of three technical replicates. Unpaired t test was applied for comparison between two groups. Data comparison among multiple groups was performed using one-way ANOVA with Tukey’s post hoc test. Data comparison between groups at different time points was performed using repeated-measures ANOVA with Bonferroni correction.