Fig. 1.

An unbiased 3D model of the C. albicans cell wall and outer fibrils. A. A single, 2D slice from the optimised tomogram. The distinct layers of the cell wall are indicated. B. Projection of the reconstructed 3D model of the cell wall. The process for generation of the unbiased 3D model (panel B) from the tomogram (panel A) is shown in video 2. C. A sector of the cell wall model. The layers have been segmented out individually and rendered in different colours, as indicated. D. The inner layer of the wall is a dense but regular, highly interconnected network of fibrils (shown in 3D in video 3). E. Fibril shafts decorated with multiple fan-like lateral branches form the outer fibrillary layer of the cell wall. In all panels, the scale bars represent 20 nm. For 3D images, the x, y and z axes are shown in red, green and blue.