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. 2020 Nov 8;6:100047. doi: 10.1016/j.tcsw.2020.100047

Fig. 2.

Fig. 2

The distribution of chitin, β(1,3)-glucan and mannan in the C. albicans cell wall. A. Electron micrographs of wild type (CAI-4 + CIp10) and chs3Δ cells stained with the ChBD-reporter/anti-HuCκ Ab/anti-mouse IgG-gold. B. Electron micrographs of sections of wild type (CAI-4 + CIp10), och1Δ, pmr1Δ and chs3Δ cells stained with Fc-dectin-1/anti-human IgG-gold. C. Electron micrographs of wild type (CAI-4 + CIp10) and och1Δ cells stained with anti-mannan scAb/anti-HuCκ Ab/anti-mouse IgG-gold. The mannan competition was performed by mixing the scAb with purified mannan before staining sections. The secondary (2°) and tertiary (3°) antibody-only control was performed by staining sections with anti-HuCκ Ab/anti-mouse IgG-gold only. Scale bars represent 0.2 μm and are the same for all images in panels A and B, and as indicated on the images in panel C. Diagrams of the antibodies/reporters are provided to the right of each panel. ChBD – chitin binding domain; HuCκ – human kappa light chain; 6 × His – hexahistidine tag; CRD – carbohydrate recognition domain and stalk region of dectin-1; Fc - Human IgG1 Fc region; scFv – single chain variable fragment (mannan-binding domain).