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. 2020 Nov 8;6:100047. doi: 10.1016/j.tcsw.2020.100047

Fig. 4.

Fig. 4

Treatment with enzymes that degrade cell wall components alters the C. albicans cell wall ultrastructure. A-H. Electron micrographs of cell walls of wild type (CAI-4 + CIp10) cells treated with (+) and without (-) enzyme for 2 h at 37 °C. ± Endo H (A, B), ± pronase (C, D), ± α(1–2,3,6)-mannosidase from Jack Bean (E, F), ± α(1–2)-mannosidase from Aspergillus saitoi (G, H). I-L. Electron micrographs of cell walls of wild type (CAI-4 + CIp10) cells treated sequentially with (+) and without (-) Endo H then with (+) and without (-) pronase for 2 h at 37 °C. M−P. Electron micrographs of cell walls of mutant strains treated with (+) and without (-) pronase for 2 h at 37 °C. mnt1-mnt2Δ ± pronase (M, N), pmr1Δ mutant ± pronase (O, P). The scale bars represent 0.2 μm and are the same for all panels.