Table 3.
Distribution of gold-particles in the layers of the cell wall.
| Reporter | C. albicans strain | Outer (fibrillar layer) | Inner (between fibrils and membrane) | Cell membrane | (total gold particles counted) | Average gold particles per cell wall* | (cell walls) |
|---|---|---|---|---|---|---|---|
| ChBD-reporter | wild type | 7.4% | 78.3% | 14.2% | (n = 351) | 4.2 | (n = 83) |
| ChBD-reporter + 5x chitin | wild type | 31.8% | 36.4% | 31.8% | (n = 44) | 1.4 | (n = 32) |
| ChBD-reporter + 10x chitin | wild type | 0.0% | 100.0% | 0.0% | (n = 1) | 0.1 | (n = 19) |
| ChBD-reporter | chs3Δ | 36.4% | 63.6% | 0.0% | (n = 11) | 0.4 | (n = 26) |
| anti-HuCκ, anti-mouse IgG-gold | wild type | 0.0% | 0.0% | 100.0% | (n = 4) | 0.1 | (n = 42) |
| Irrelevant Hap2 scAb | wild type | 0.0% | 70.0% | 30.0% | (n = 10) | 0.9 | (n = 11) |
| Fc-dectin-1 | wild type | 18.5% | 64.3% | 17.2% | (n = 1151) | 14.9 | (n = 77) |
| anti-human IgG-gold | wild type | 0.0% | 0.0% | 0.0% | (n = 0) | 0.0 | (n = 19) |
| Fc-dectin-1 | och1Δ | 19.6% | 71.0% | 9.3% | (n = 535) | 24.3 | (n = 22) |
| Fc-dectin-1 | och1Δ re-integrant | 17.4% | 64.6% | 18.1% | (n = 144) | 7.2 | (n = 20) |
| Fc-dectin-1 | pmr1Δ | 24.1% | 64.0% | 11.9% | (n = 303) | 11.2 | (n = 27) |
| Fc-dectin-1 | pmr1Δ re-integrant | 21.2% | 59.4% | 19.4% | (n = 520) | 11.1 | (n = 47) |
| Fc-dectin-1 | chs3Δ | 22.0% | 67.5% | 10.5% | (n = 909) | 33.7 | (n = 27) |
| anti-mannan scAb | wild type | 48.2% | 21.2% | 30.6% | (n = 353) | 13.1 | (n = 27) |
| anti-mannan scAb + mannan | wild type | 0.0% | 8.6% | 91.4% | (n = 35) | 2.9 | (n = 12) |
| mannan | wild type | 0.0% | 0.0% | 0.0% | (n = 0) | 0.0 | (n = 11) |
| anti-mannan scAb | och1Δ | 20.8% | 24.5% | 54.7% | (n = 106) | 8.2 | (n = 13) |
| anti-mannan scAb | pmr1Δ | 0.0% | 0.0% | 100.0% | (n = 5) | 0.3 | (n = 15) |
| anti-mouse IgG-gold | wild type | 33.3% | 66.7% | 0.0% | (n = 6) | 0.5 | (n = 13) |
*see Fig. S2 for an example of this calculation.