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. 2020 Nov 4;101(6):215–222. doi: 10.1111/iep.12374

FIGURE 3.

FIGURE 3

GAS5 overexpression inhibits M2‐like polarization of TAMs by regulating PTEN expression. TAMs were transfected with pcDNA3.1‐GAS5 or pcDNA3.1‐NC and si‐PTEN or si‐Ctrl. The expression of TNF‐α (A), IL‐12 (B), IL‐10 (C) and IL‐13 (D) in the modified TAMs was detected by qRT‐PCR. SMCC‐7721 cells were incubated with cell supernatant of the modified TAMs for 24 or 48 h. E, MTT assay was performed to explore the cell proliferation of SMCC‐7721 cells. F, Transwell assay was performed to detect the cell invasion of SMCC‐7721 cells. (*P < .05 compared with the Vector + si‐Ctrl group, **P < .01 compared with the Vector + si‐Ctrl group, ## P < .01 compared with the Vector + si‐PTEN group)