Table 3.
Antibodies and fluorescent reagents used in flow cytometry.
| Antibody | Fluorochrome | Vendor | No./Clone | |
|---|---|---|---|---|
| Th cells | Anti-human CD4 | FITC | eBioscience | 11-0049-42/RPA-T4 |
| Anti-human T-bet | PerCP-Cy5.5 | eBioscience | 45-5825-80/eBio4B10 | |
| Isotype mIgG1 | PerCP-Cy5.5 | eBioscience | 45-4714-82 | |
| Anti-human GATA-3 | APC | eBioscience | 50-9966-41/TWAJ | |
| Isotype rIgG2b | APC | eBioscience | 50-4031-82 | |
| Anti-human ROR-ɣt | PE | eBioscience | 12-6988-80/AFKJS-9 | |
| Isotype rIgG2a | PE | eBioscience | 12-4321-80 | |
| Anti-human IFN-γ | PerCP-Cy5.5 | eBioscience | 45-7319-41/4S.B3 | |
| Isotype rIgG2b | PerCP-Cy5.5 | eBioscience | 45-4031–80 | |
| Anti-human IL-4 | APC | eBioscience | 17-7049-42/8D4-8 | |
| Isotype mIgG1 | APC | eBioscience | 17-7049-42 | |
| Anti-human IL-17 | PE | eBioscience | 12-7178-42/64CAP17 | |
| Isotype mIgG1 | PE | eBioscience | 12-4714-82 | |
| Tregs | Anti-human CD4 | FITC | eBioscience | 11-0049-42/RPA-T4 |
| Anti-human CD25 | PE | eBioscience | 12-0259-41/BC96 | |
| Anti-human CD127 | APC | eBioscience | 17-1278-41/eBioRDR5 | |
| B cells | Anti-human CD19 | APC | eBioscience | 17-0199-41/HIB19 |
| Anti-human IgD | FITC | eBioscience | 11-9868-41/IA6-2 | |
| Anti-human CD27 | PE | eBioscience | 12-0271-81/LG.7F9 | |
| Tfh cells | Anti-human CD4 | PerCP-Cy5.5 | eBioscience | 35-0047-41/SK3 |
| Anti-human PD1 | PE | eBioscience | 12-2799-41/eBioJ105 | |
| Anti-human CXCR5 | FITC | eBioscience | 11-9185-41/MUSUBEE |