Figure 11.

Cytoskeletal filaments are not modified by AnkF expression. HeLa cells were transiently transfected with pGFP alone or with pEGFP-AnkF (green). 24 h post-transfection, cells were fixed and stained with Phalloidin-Alexa647 for actin, or with tubulin- and cytokeratin 18-specific antibodies by indirect immunofluorescence (red). Nuclei and bacterial DNA were stained with DAPI (blue). Cells were visualized using LSM. Representative images from two independent experiments are shown.