Figure 5.

The AnkF transposon mutant replicates inside a CCV harboring wild-type bacteria. HeLa cells were infected with C. burnetii expressing IPTG-inducible tdTomato (WT-tdT) in the presence of 2 mM IPTG and/or ankF::Tn (ankF::Tn) endogenously expressing GFP at an MOI of 50 each as indicated. At 72 h post-infection, the cells were fixed and nuclei and bacterial DNA was stained with DAPI (blue). Representative LSM images of two independent experiments are shown. A red box represents a close-up without DAPI staining of the merged image. White arrows indicate single ankF::Tn cells.