Figure 6.

The replication defect of ΔankF C. burnetii can be complemented. HeLa cells were infected with C. burnetii wild-type (WT), ΔankF or ΔankF::AnkF at an MOI of 100. At 60 h post-infection, the cells were fixed and stained with an anti-LAMP2 antibody (green) and with antisera against C. burnetii (red). Nuclei and bacterial DNA were stained with DAPI (blue). (A) Representative LSM images of three independent experiments are shown. (B) The percentage of infected cells were determined by analyzing 100 cells per experiment in three independent experiments. Error bars indicate ± SD. **p < 0.01, ***p < 0.001.