Figure 2.

Time-Dependent Effect of TRV027 on ECG, Plasma Concentrations of Adrenaline and Noradrenaline, and the Serum Concentration of Aldosterone After Administration of Saline or TRV027

(A) Electrocardiography (ECG) was recorded indicated time after injection of TRV027 (3 mg/kg) to neonatal mice (left column). Then, 10 min after additional injection of atropine + propranolol, ECG was once again recorded (right column). (B) Each parameter of ECG was measured different time after injection of TRV027 (3 mg/kg) (open circles). Then, 10 min after injection of atropine + propranolol, each parameter was measured again (closed circles). No significant time-dependent change in each parameter was detected with 1-way analysis of variance (ANOVA) followed by Dunnett’s test. Two-way ANOVA indicated a significant effect of drugs in heart rate (HR), P, and PQ but no significant interaction between time and drugs. †p < 0.05; ††p < 0.01; and †††p < 0.001 between saline and atropine + propranolol groups. n = 5 in each group. (C) Plasma adrenaline and noradrenaline concentrations 2 and 8 h after TRV027 (3 mg/kg) administration. The p value was calculated with 1-way ANOVA followed by Dunnett’s test. n = 5 to 11 in each group. (D) Serum aldosterone concentration 8 h after saline or TRV027 (3 mg/kg) administration. The p value was calculated with unpaired Student’s t test. n = 5 to 6 in each group. Abbreviations as in Figure 1.