Figure 3.

Effect of TRV027 on Twitch Ca²⁺ Transients of Isolated mNVCMs or hiPSC-CMs

Representative twitch Ca²⁺ transients of (A) mouse neonatal ventricular cardiac myocytes (mNVCMs) detected with Fluo-4 and digitized at 144 Hz or (B) human induced pluripotent stem cell–derived cardiac myocytes (hiPSC-CMs) detected with Cal-520 and digitized at 60 Hz 2 h after in vitro treatment with saline (black line) or TRV027 (3 μmol/l) (red line). Action potential was elicited by field stimulation at 0.33 Hz. (C) Effects of saline or TRV027 on the peak ΔF/F₀, time to peak (TTP), half decay time (T_1/2), and the sarcoplasmic reticulum Ca²⁺ content in paced mNVCMs. n = 10 in each group. (D) Effects of saline or TRV027 on the peak ΔF/F₀, TTP, and a half decay time of paced hiPSC-CMs, and their spontaneous beating rate in the absence of pacing. n = 8 to 11 in each group. Statistical significance was analyzed with unpaired Student’s t test.