Figure 4.

Effect of TRV027 on the Mitochondrial OCR in mNVCMs

(A) Oxygen consumption rate (OCR) in isolated mouse neonatal ventricular cardiac myocytes (mNVCMs) treated in vitro with saline or TRV027 (3 μmol/l) for 2 h. Oligomycin A (2 μmol/l), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) (2 μmol/l), and antimycin A + rotenone (1 μmol/l each) were sequentially added as indicated. Letters in this panel (a, b, and c) indicate the timing at which parameters shown in B were calculated. (B) Unloaded and maximum OCRs calculated by subtracting OCR after antimycin A and rotenone (c in A) from that before oligomycin (a in A) or after FCCP (b in A), respectively. (C) Measurement of OCR in mNVCMs treated in vitro for 2 h with saline or TRV027 (3 μmol/l) and with nifedipine (10 μmol/l) for last 30 min. (D) Unloaded and maximum OCR in the presence of nifedipine. (E) Short term effect of wash-in of saline or TRV027 (3 μmol/l) on unloaded OCR in mNVCMs. Saline or TRV027 was added at indicated this time point while OCR was measured. (F) Time-dependent effect of saline or TRV027 on unloaded OCR for ∼35 min after their wash-in. Statistical significance was analyzed with unpaired Student’s t test. n = 5 to 8 in each group.