Figure 4.

pSMAD1 Connects SCUBE1 Expression to BMPR2 Signaling

PAECs were treated with (si-NC, si-SCUBE1, Lenti-GFP, or Lenti-SCUBE1. (A) BMPR2 mRNA levels were determined by reverse transcription, quantitative polymerase chain reaction (n = 4 samples per group). (B–D) Immunoblotting was performed to quantify for phosphorylated fraction of Smad1/5/9 (pSMAD1) and of Smad2/3 (pSMAD2) as well as total SMAD1 and SMAD2 (B). Ratios of phosphorylated to total SMAD1/5/9 (n = 3 samples per group) (C) and SMAD2/3 (n = 3 samples per group) (D) were determined by densitometric analysis. Immunoblots are representative of 3 independent experiments. Values are presented as mean ± SD. The p values were calculated by Student's t-test. The comparisons with p > 0.05 were not explicitly stated in the panels. Abbreviations as in Figures 1 and 2.