Figure 5.

Scube1 Expression Is Decreased in Rodent Models of PAH

Male Sprague-Dawley rats were injected with monocrotaline (MCT) (versus vehicle control [Ctl]) or injected with SU5416 (SuHx) and exposed chronically to 10% O₂ for 3 weeks followed by 2 weeks of normoxia (vs. vehicle Ctl in normoxia). Male C57BL/6 interleukin (IL)-6 transgenic mice were subjected to 3 weeks of hypoxia (10% O₂) exposure (IL6/Hx) versus normoxia Ctl. Acute bacterial pneumonia (PNA) was generated in male and female C57/BL mice at 0 h (baseline Ctl) and after 48 h following intratracheal administration of K. pneumoniae. Acute myocardial infarction (AMI) was induced by direct ligation of left coronary artery (vs. sham surgery Ctl) for 5 days in C57BL/6 mice. Scube1 protein levels were determined by enzyme-linked immunosorbent assay in plasma (n = 3 to 7 per group) (A,C,E,G,I) and lung or heart tissue homogenate (n = 3 to 6 per group) (B,D,F,H,J) collected from euthanized animals. Values are presented as mean ± SD. The p values were calculated by Student's t-test. PAH = pulmonary arterial hypertension.