Table 3.

Mutations identified by WGS of Mtb mutants spontaneously resistant to selected compounds.

Compound	Gene	Mutation/s	Product
127-17R SRM-1	katG fadD26	*741R G74*	Catalase-peroxidase-peroxynitritase Fatty-acid-AMP ligase
127-21R SRM-1	katG ppsC Rv3755c	Y597N; S315Na Q1085* D100G	Catalase-peroxidase-peroxynitritase Phenolpthiocerol synthesis type-I polyketide synthase Unknown (conserved protein)
127-21R SRM-2	katG	S315R	Catalase-peroxidase-peroxynitritase
127-23R SRM-1	katG ppsB	V544F Q945P	Catalase-peroxidase-peroxynitritase Phenolpthiocerol synthesis type-I polyketide synthase
127-23R SRM-2	katG ppsC phoP	G285V +c insertion at nt 680 IS6110 insertion at nt 210	Catalase-peroxidase-peroxynitritase Phenolphthiocerol synthesis type-I polyketide synthase Possible two component system response transcriptional positive regulator PhoP
127-13R SRM-1 127-13R SRM-2	Rv0678 fadD26 Rv3479	-g deletion at nt 198 S553* N831K	Regulator of MmpS5-MmpL5 efflux pump Fatty-acid-AMP ligase Transmembrane protein
127-15R SRM-1 127-15R SRM-2	Rv2466c fadD26	-t deletion at nt 151 G74*	Mycothiol dependent reductase, D Fatty-acid-AMP ligase

*stop codon; nt, nucleotide.

^aSince these sites were heterogeneous, 127-21^R SRM-1 was probably a mixture of 2 mutants, each with a different KatG mutation.