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. 2020 Nov 26;10:20645. doi: 10.1038/s41598-020-77743-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Optimisation of electroporation parameters in B16F10 cells. B16F10 cells were electroporated at a range of concentrations and cell poration was measured by PI uptake during EP (a). Cell viability was determined by PI permeability 30 min following treatment. Cells were electroporated at 700 V/cm with or without drug and were analysed for viability 24, 48 and 72 h post treatment (b). Data shown is representative of an n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001 compared to NT unless specified.