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. 2020 Nov 26;10:20645. doi: 10.1038/s41598-020-77743-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic evaluation of melanoma conditioned BMDMs following electroporation with bleomycin or calcium. Melanoma conditioned BMDMs were electroporated in the presence of bleomycin or calcium and cultured for 72 h. Cells were analysed by flow cytometry (a,b) for the macrophage activation markers F4/80, CD115 and CD206. BMDMs were identified as CD11b⁺ and gated according to F4/80, CD115 and CD206 expression. Intracellular arginase activity was assayed following cell lysis (c). Data shown is representative of an n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001 compared to NT or EP only as appropriate unless specified.