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. 2020 Nov 26;10:20645. doi: 10.1038/s41598-020-77743-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of melanoma conditioned BMDMs following electroporation on CD4⁺ T cells. Melanoma conditioned BMDMs were electroporated in the presence of bleomycin or calcium and cultured for 24 h. Cells were then cocultured with freshly isolated CFSE labelled CD4⁺ T cells. T cell proliferation was measured by reduced CFSE expression (a). Levels of IL-4 (b), IL-10 (c) and IFNγ (d) in the supernatant were measured by ELISA. Activated T cells were gated by low CFSE expression and CD25 (e) and CXCR3 (f) expression was determined by flow cytometry. Data shown is representative of an n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001 compared to NT or EP only as appropriate unless specified.