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. 2020 Nov 13;8:565782. doi: 10.3389/fchem.2020.565782

Figure 11.

Figure 11

Polydiacetylene-based Raman probes for targeted live-cell Raman imaging. (A) Topochemical polymerization for the preparation of PDDA. (B) Overlaid Raman spectra of individual DMSO solution of PDDA and a series of representative Raman probes, including 1. ethynylbenzene; 2. 5-ethynyl-2′-deoxyuridine; 3. diphenyl phosphorazidate; 4. diphenylbutadiyne; 5. benzonitrile; 6. 5-bromopentanenitrile; 7.deca-4,6-diynedioic acid. (C) Schematic illustration of side chain modification of PDDA for subcellular organelle targeting Raman imaging. (D) Side chain modification of PDDA with different functional groups to derivatives P2, P3, and P4 that can be targeted to specific subcellular organelles. (E) Images of HeLa cells treated with 50 μM of P2, P3, and P4, respectively. Images shown from left to right are the alkyne (2,120 cm−1), lipids (2,850 cm−1), and merged images. Reprinted with permission from Tian et al. (2020). Copyright 2020, Nature.