Fig. 4.

hGC33-null-NP and hGC33-induced cell cycle arrest in G1 phase and inhibited cyclinD1 expression in HepG2 cells. a Representative cell–cycle diagram of various groups treated with hGC33-null-NP and hGC33. b Cell cycle analysis of various groups treated with hGC33-null-NP and hGC33. The HCC cells were incubated with 0.5 μm hGC33 or hGC33-null-NP (with the molar concentration of hGC33 as reference). *P < 0.05, the G1 phase of hGC33-null-NP or hGC33 cells was compared with that of 0 h cells. c, d After hGC33-null-NP or hGC33 treatment, cyclinD1 was significantly down regulated in HepG2 cells compared with that in the control group