FIGURE 4.

Lrp1 deletion increases the number of newborn ASPA⁺ OLs in the adult mouse corpus callosum and motor cortex. (A–L) Confocal images from the corpus callosum (CC; A–F) or motor cortex (Ctx; G–L) of P57+30 control (Pdgfrα-CreER^TM:: Rosa26-YFP) and Lrp1-deleted (Pdgfrα-CreER^TM:: Rosa26-YFP:: Lrp1^fl/fl) mice that were immunolabeled to detect ASPA (red), YFP (green), and the nuclear marker Hoechst 33342 (HST, blue). Solid yellow arrowheads indicate YFP⁺ ASPA⁺ mature OLs. Solid white arrowheads indicate YFP⁺ ASPA-neg cells. (M) Quantification of the proportion (%) of YFP⁺ cells that are YFP⁺ ASPA⁺ newborn mature OLs in the CC and Ctx of P57+30 control and Lrp1-deleted mice [mean ± SD for n = 4 mice per genotype; 2-way ANOVA: Genotype F (1, 12) = 125.3, p < 0.0001; Brain region F (1, 12) = 42.1, p < 0.0001; Interaction F (3, 28) = 4.5, p = 0.056]. Bonferroni multiple comparisons: *p < 0.05, ***p < 0.001. Scale bars represent 30 μm (A–J).