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. 2020 Oct 29;1(8):100137. doi: 10.1016/j.xcrm.2020.100137

Figure 4.

Figure 4

In Vivo, R788 Reduces Excess MUC1 from Lung Epithelia of Mice with ALI

(A) IF images from lung tissue sections stained with MUC1 (green), phalloidin (yellow), and DAPI (gray) demonstrate that ischemia/reperfusion (I/R)-induced remote ALI resulted in increased MUC1 in lung epithelium. Treatment with fostamatinib over the course of 10 days suppressed MUC1 levels in mouse lung epithelium.

(B) Single-cell tissue analysis, based on IF of MUC1 and phalloidin (panel 1). In each image (panel 1), nuclei were identified based on DAPI staining (rainbow colors represent different cell nuclei in panel 2). The cell bodies were identified based on phalloidin staining surrounding each nucleus (orange cell borders, panel 3). Lastly, MUC1 IF intensity (green in panel 3) and phalloidin intensities were calculated within each cell body.

(C) Bar graph ratio of MUC1:phalloidin intensities in all cells of tissue sections from sham-treated mice and mice subjected to I/R-induced ALI, treated either with or without fostamatinib. Average MUC1 intensity values per cell were normalized to the average phalloidin levels. Means ± SDs (n = 3 mice/condition/dose). ns, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.