Figure 3.

Subsets of Fetal Skin CD4⁺ Tconv and CD8⁺ T Cells Display a Memory Phenotype and Demonstrate Capacity for IFNγ Production

Cells were isolated from second trimester fetal skin (scalp and/or torso) as well as adult (torso) skin and analyzed by flow cytometry.

(A) Representative plots demonstrating CD45RO expression by fetal CD8⁺ T cells (gated on live CD3⁺CD8⁺CD4^neg) and CD4⁺ Tconv (gated on live CD3⁺CD4⁺CD8^negFoxp3^negCD25^lo).

(B and C) Percentage of CD45RO⁺ (B) CD8⁺ T cells and (C) CD4⁺ Tconv in fetal versus adult skin.

(D–F) Representative histogram (D) and quantification (E and F) of Nur77 MFI on fetal skin CD45RO⁺ versus CD45RA⁺ CD4⁺ Tconv and CD8⁺ cells.

(G–J) Percentage of CD4⁺ Tconv producing (G) IL-17A, (H) IL-13, and (I) IFNγ after PMA/ionomycin re-stimulation, and (J) percentage of IFNγ-producing CD8⁺ T cells.

Each point in (B)–(G) represents data from an individual tissue sample; for some fetal samples data from scalp and torso skin from the same fetal donor are included as separate points. ns, not significant (p > 0.05); ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.