REAGENT or RESOURCE	SOURCE	IDENTIFIER
Flow Cytometry Antibodies
Anti-human CD3-BV711 (clone: SK7)-80ug/ml	Biolegend	Cat# 344838; RRID:AB_2565827
Anti-human CD4-PE -Texas Red(clone: MHCD0417)	Life Technologies/ Thermo Fisher Scientific	Cat# MHCD0414; RRID: 10371766
Anti-human CD8-BV650 (clone: SK1) 100ug/ml	Biolegend	Cat# 344730; RRID:AB_2564510
Anti-human CD25-PECy7 (clone: M-A251)	BD Biosciences	Cat# 557741; RRID:AB_396847
Anti-human CD27- PerCP-e710 (clone: 0323)	eBioscience	Cat# 46-0279-42; RRID:AB_1834391
Anti-human CD31- APC-e780 (clone: WM59)	eBioscience	Cat# 47-0319-42; RRID:AB_10730582
Anti-human CD45-APC (clone: HI30)	eBioscience	Cat# 47-0459-41; RRID:AB_1944369
Anti-human CD45RA-BV605(clone: HI100) 25ug/ml	Biolegend	Cat# 304134; RRID:AB_2563814
Anti-human CD45RO-FITC (clone: UCHL1)	eBioscience	Cat# 11045742; RRID:AB_10596999
Anti-human IL2-PE 25ug/ml (MQ1-17H12)	Biolegend	Cat# 500307; RRID:AB_315094
Anti-human CD152/CTLA4-PE (clone: 14D3)	Thermo Fisher Scientific	Cat# 12-1529-42; RRID:AB_10805626
Anti-human CD278/ICOS-APC (clone: ISA-3)	eBiosceince	Cat# 17-9948-42; RRID:AB_10598209
Anti-human FoxP3-e450 (clone: PCH101)	eBioscience	Cat# 48477642; RRID:AB_1834364
Anti-human IFNγ− A700(clone: 4S.B3)	Biolegend	Cat# 502520; RRID:AB_528921
Anti-human IL-13 –FITC (clone: 85BRD)	Thermo Fisher Scientific	Cat# 11-7136-42; RRID: 2572515
Anti-human IL-17A-APC ef-780 (clone: eBio64CAP17)	eBioscience	Cat# 47-7179-41; RRID:AB_11043274
Anti-human CD141-APC (Clone:BDCA-3)	Milteny Biotech	Cat# 130-113-314; RRID:AB_2733313
Anti-human EpCAM-BV711 (9CY)	Biolegend	Cat# 324240; RRID:AB_2734307
Anti-human CD11c-BV605 (B-Ly6)	BD BioSciences	Cat# 563929; RRID:AB_2744276
Anti-human Ki-67- PerCyp Cy5.5 (clone: B56)	BD Biosciences	Cat# 561284; RRID:AB_10611574
Anti-human IL-22-PE (clone: 142928)	R & D Systems	Cat#IC7821P; RRID:AB_495011
Anti-human TNFα−PE-Cy7 (clone: Mab11)	BD BioSciences	Cat#557647; RRID: AB_396764
Anti-human CD14-PE-200ug/ml (MFE2)	Biolegend	Cat# 301806; RRID:AB_314188
Anti-human CD16-BV421 (3G8)	Biolegend	Cat# 302038; RRID:AB_2561578
Anti-human CD1c-PECy7 100ug/ml (clone: L161)	Biolegend	Cat# 331516; RRID:AB_2275574
Anti-human CD19-APC (SJ25C1)	Tonbo Bioscience	Cat# 20-0198-T100; RRID: 2621683
Anti-human CD24-BV711 (ML5)	BD Biosciences	Cat# 563371; RRID:AB_2738164
Anti-human HLA-DR –PercP Cy5.5(clone: Tu36) Anti-human HLA-DR-BV 650 (L243)	Biolegend Biolegend	Cat# 361608; RRID:AB_2563198 Cat# 307649; RRID:AB_2562544
Anti-human CD1a-AF700 (H1149)	Biolegend	Cat# 300120; RRID:AB_528764
Anti-mouse Nur77-PE (12.14)	eBioscience	Cat# 12-5965-82; RRID:AB_1257210
Mass Cytometry Antibodies
Anti-human CD45 (clone: H130, Metal: 89Y)	Fluidigm	Cat# 3089003B; RRID: AB_2661851
Anti-human CD19 (clone: HIB19, Metal: 142Nd)	Fluidigm	Cat# 3142001B; RRID: AB_2651155
Anti-human CD11b (clone: ICRF44, Metal: 144Nd)	Fluidigm	Cat# 3144001B; RRID: AB_2714152
Anti-human CD4 (clone: IRPA-T4, Metal: 145Nd)	Fluidigm	Cat# 3145001B; RRID: AB_2661789
Anti-human CD11c (clone: BU15, Metal: 147Sm)	Fluidigm	Cat# 3147008B; RRID: AB_2687850
Anti-human CD25 (clone: 2A3, Metal: 149Sm)	Fluidigm	Cat# 3149010B; RRID: AB_2756416
Anti-human γδTCR (clone: 11F2, Metal: 152Sm)	Fluidigm	Cat# 3152008B; RRID: AB_2687643
Anti-human CD8α (clone: RPA-T8, Metal: 53Eu)	Biolegend	Cat# 301002; RRID: AB_2661818
Anti-human CD24 (clone: ML5, Metal: 154Sm)	Biolegend	Cat# 311102; RRID:AB_10580644
Anti-human CD56 (clone: B159, Metal: 155Gd)	Fluidigm	Cat# 3155008B; RRID: AB_2861412
Anti-human CD27 (clone: L128, Metal: 158Gd)	Fluidigm	Cat# 3158010B; RRID: AB_2858231
Anti-human PDL1 (clone: 29E.2A3, Metal: 159Tb)	Fluidigm	Cat# 3159029B; RRID:AB_2861413
Anti-human CD152/CTLA4 (clone: 14D3, Metal: 161Dy)	Fluidigm	Cat# 3161004B; RRID: AB_2687649
Anti-human FoxP3 (clone: PCH101, Metal: 162Dy)	Fluidigm	Cat# 3162011A; RRID: AB_2687650
Anti-human CD1c-PECy7 (clone: L161, Metal: 164Dy)	Biolegend	Cat# 331502; RRID: AB_2661820
Anti-human PD1 (clone: EH12.2H7, Metal: 166Er)	Biolegend	Cat# 329902; RRID: AB_940488
Anti-human Ki-67 (clone: Ki67, Metal: 168Er)	Biolegend	Cat# 3168001B; RRID: AB_2810856
Anti-human CD3 (clone: UCHT1, Metal: 170Er)	Fluidigm	Cat# 3170001B; RRID: AB_2811085
Anti-human CD45RO (clone: UCHL1, Metal: 173Yb)	Fluidigm	Cat# 3173016D; RRID: AB_2811052
Anti-human CD207/Langerin (clone: 4C7, Metal: 175Lu)	Fluidigm	Cat# 3175016B; RRID:AB_2861414
Anti-human CD127 (clone: A019D5, Metal: 176Yb)	Fluidigm	Cat# 3176004B; RRID: AB_2687863
Immunohistochemistry Antibodies
Anti-human CD4 (clone: 41B2)	Agilent	Cat# IS64930-2; RRID:AB_2861415
Anti-human FoxP3 (clone: 236A/E7)	Abcam	Cat# ab20034; RRID: AB_445284
Biological Samples
De-identified healthy adult and fetal skin tissue	UCSF-affiliated hospitals	N/A
Chemicals, Peptides, and Recombinant Proteins
Collagenase from Clostridium histolyticum, Type IV	Sigma-Aldrich	Cat# lS00418
DNase	Sigma-Aldrich	Cat# DN25
Fetal bovine serum	Fisher scientific	Cat# SH3054103
Ghost Dye™ Violet 510 Live/Dead Stain	Tonbo Biosciences	Cat#13-0870-T100
FoxP3-staining buffer kit	Invitrogen by Thermofisher	Cat# 00-5523
TONBO cell stimulation cocktail	Tonbo Biosciences	Cat# TNB-4975
Cisplatin	Sigma-Aldrich	Cat# P4394
Intercalator-IR	Fluidigm	Cat# P4394
Cell-ID 20-Plex Pd Barcoding Kit	Fluidigm	Cat# 201060
BOND Polymer Refine Detection Kit	Leica Biosystems	Cat# DS9800
Deposited Data
Raw data	This paper	Flow repository: ID# FR-FCM-Z3YD (url: https://flowrepository.org/)
Software and Algorithms
GraphPad Prism	GraphPad Software, Inc	https://www.graphpad.com:443/scientific-software/prism/
FlowJo v10.5.0	FlowJo, LLC	https://www.flowjo.com/solutions/flowjo
R Statistical Computing Software	The R Foundation; https://www.r-project.org/	https://www.r-project.org/
CyTOF Workflow	Nowicka et al., 2017	https://www.bioconductor.org/packages/release/workflows/html/cytofWorkflow.html