Figure 4.

Transcriptome Deconvolution Reveals B Cell Enrichment in Tumors of Responding Patients

(A) Fusion analysis utilizing transcriptome data revealed an enrichment in immunoglobulin rearrangements in tumors of responding patients, which was reflective of a higher pre-existing intratumoral B cell infiltration (Mann Whitney p = 7e−04).

(B) Through gene expression signature analysis and deconvolution of RNA sequence data, we identified an enrichment in tumor associated B cells in baseline tumors of responding patients (Mann Whitney p = 9.3e−05).

(C and D) This observation was driven by enrichment in the naive B cell and plasma cell populations (Mann Whitney p = 0.013 and p = 0.03, respectively).

(E) Through unsupervised clustering of relative abundance of 22 immune-cell-type populations, patients with clinical response to therapy clustered together and showed higher relative abundance in B cell subsets as well as CD8+ T cells. Z scores were computed across samples for each immune cell type separately using the relative abundance measurements obtained from CIBERSORT.