REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-CD68 Monoclonal Antibody	Abcam	Cat# ab955, RRID:AB_307338
Rabbit anti-Osteopontin antibody	Abcam	Cat# ab8448, RRID:AB_306566
Mouse anti M-CSF R antibody	R & D Systems	Cat# MAB3291, RRID:AB_2292260
IL6R antibody (Tocilizumab)	Roche	N/A
Rabbit anti-Albumin Antibody	Sigma-Aldrich	Cat# A0433, RRID:AB_257887
Goat anti Osteopontin antibody	R & D Systems	Cat# AF1433, RRID:AB_354791
Biological Samples
Human Serum samples	University of Michigan	N/A
Human Serum samples	Genentech clinic trial for Tociilizmab	FaSScinate(NCT01532869)/FcuSSced (NCT02453256) cohorts
Human blood samples	Genentech	N/A
Human healthy lung samples	Northern California Transplant Donor Network	N/A
Human SSc lung samples	UCSF	N/A
Chemicals, Peptides, and Recombinant Proteins
Recombinant human OPN	Sigma-Aldrich	SRP3131
Human serum albumin	Sigma-Aldrich	A9080
Recombinant human IL6	R & D Systems	206-IL
Recombinant human MCSF	R & D Systems	216-MC
Critical Commercial Assays
Human Osteopontin Quantikine ELISA Kit	R & D Systems	DOST00
Simple Plex Cartridge Kit for OPN	Bio Techne	SPCKB-PS-000232
Bio-Plex Pro Human Cytokine 48-plex assay	Bio Rad	12007283
CellTiter-Glo Luminescent Cell Viability Assay	Promega	G7570
Chromium Single Cell 3′ Library and Gel bead kit v2	10x Genomics	PN-120267
Pan monocytes isolation kit, human	Miltenyi Biotec	130-096-537
Deposited Data
RNA seq for IC stimulated monocytes	GSE155687	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE155687
scRNA seq for fibrotic lung	GSE159354
Experimental Models: Cell Lines
Primary human lung fibroblast cells	ATCC	PCS201013
Software and Algorithms
JMP	SAS Institute	SCR_014242
Prism 7	GraphPad	SCR_005375
Seurat R package version 3.1.1		https://satijalab.org/seurat