Figure 1.

Stratification of PDAC Tumors According to Their Energetic Metabolism

(A) Oxygen consumption rate (OCR) was measured in the 6 classical PDAC cell lines under basal conditions and following the injection of oligomycin, carbonyl cyanide-p-trifluoromethoxyphenyl-hydrazon (FCCP), and rotenone + antimycin A.

(B) Extracellular acidification rate (ECAR) was measured under basal conditions and following the addition of glucose, oligomycin, and 2-deoxyglucose.

(C) Basal OCR versus basal ECAR plot (both normalized to 10,000 seeded cells) shows the clustering of the 6 PDAC classical cell lines into 3 metabolic groups: Panc-1 is mostly respiratory (OXPHOS); MIA PaCa-2 and BxPC-3 are mostly glycolytic (glycolytic); and Capan-1, Capan-2, and to a lesser extent SOJ-6, have a relative lower energetic metabolism compared to the other cell lines (less metabolic group). The dotted lines indicate the thresholds that we selected between high and low OCR and ECAR.

(D) Basal OCR versus basal ECAR plot (both normalized to 10,000 seeded cells) shows the clustering of the 21 primary PDAC cancer cells derived from PDX into 4 different groups with respect to their basal energetic features. A fourth group with high rates of both OCR and ECAR was found. High OXPHOS patients are depicted with the red color, and low OXPHOS with blue color. The dotted lines indicate the thresholds that we selected between high and low OCR and ECAR. This plot is representative of at least 3 independent experiments for each patient. For clarity, a short version of the anonymized name of patients is used.

Representative profiles of at least 3 independent experiments are shown in (A) and (B).

See also Figures S1 and S2 and Table S1.