FIGURE 1.

Translational activation of maternal Ccnb1 mRNAs with short and long 3′-UTRs. (A) Schematic representation of three different forms of Ccnb1 transcripts with distrinct lengths of 3′-UTRs in mouse oocyte. Relative positions of cis-elements are indicated. (B) An illustration of microinjection and treatments to oocytes in subsequent experiments. (C,E) Fluorescence microscopy (C) and western blot analysis (E) results revealing the expression levels of Flag-Gfp-Ccnb1_{short 3′–UTR} mRNA in oocytes with different U0126 (20 μM) treatment. DDB1 was used for a control. Numbers under blot bands indicate the intensity of each band. For each set of data, more than 80 oocytes were observed. Scale bar: 100 μm. (D) The ratio of the GFP and mCherry fluorescence signals intensity in (C). Data were analyzed by mean ± SEM: *P < 0.05. (F,H) Fluorescence microscopy (F) and western blot analysis (H) data revealing the expression of Flag-Gfp-Ccnb1_{long 3′–UTR} reporter mRNA in oocytes with or without U0126. For each set of data, more than 80 oocytes were gathered. Scale bar: 100 μm. (G) The ratio of the GFP and mCherry fluorescence signals intensity in (F). n.s. indicates non-significant. DDB1 was used for a control. Numbers under blot bands indicate the intensity of each band. Data were analyzed by mean ± SEM: ***P < 0.001.