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. 2020 Nov 26;10:20621. doi: 10.1038/s41598-020-77749-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Qualitative analysis of total protein content in green, red and stored apple fruit extracted by using three different protocols: TCA/acetone precipitation with phenol extraction, EDTA/SDS extraction and phenol/SDS extraction. Bars show the standard error. Different letters indicate significant differences among the development stages (according to ANOVA with p < 0.05).