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. 2020 Nov 26;10:20621. doi: 10.1038/s41598-020-77749-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoblotting of AGPs in green, red and stored apple fruit. Total protein extract (a) and Yariv reagent-precipitated material (b) were separated in SDS-PAGE for immunoblot analysis with JIM13, LM2, and LM14. Immunoblotting relative expression [%] of AGPs in total protein extract (c) and Yariv reagent-precipitated material (d). Expression level was estimated by densitometric measurements of obtained stripes by ImageJ 1.51 software. Molecular masses are indicated on the left. The samples derive from the same experiment and blots were processed in parallel.