Figure 4.

Cellular and subcellular immunolocalization of AGPs in parenchymal cells of green, red and stored apple fruit (a), imaging using CLSM and TEM. Control reactions (b and c). Immunofluorescence of JIM13 epitope in green fruit (d), red fruit (e) and stored fruit (f). Immunofluorescence of LM2 epitope in green fruit (g), red fruit (h) and stored fruit (i). Immunofluorescence of LM14 epitope in green fruit (j), red fruit (k) and stored fruit (l). Immunogold-labelling of JIM13 epitope in green fruit (m), red fruit (n) and stored fruit (o). Bars: 50 µm for CLSM, and 500 nm for TEM. c cytoplasm, cw cell wall.