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. 2020 Nov 26;11:6000. doi: 10.1038/s41467-020-19849-9

Fig. 1. Deficiency of β-arrestin 2 decreases cellular antiviral responses.

Fig. 1

a Immunoblot of lysates of peritoneal macrophages infected with HSV-1 for indicated times. b Arrb2 mRNA levels in peritoneal macrophages infected with HSV-1 or VSV for indicated times. c, d Ifnb mRNA levels in WT or Arrb2−/− mouse peritoneal macrophages stimulated with HSV-1 (left, c) (***P < 0.0001, = 0.0006 in sequence, **P = 0.0029), ISD (right, c) (***P < 0.0001), VSV (left, d) (***P < 0.0001, < 0.0001, < 0.0001 in sequence), or SeV (right, d) (***P = 0.0003, 0.0002 in sequence, **P = 0.0070) for indicated times. e, f The virus titers as in (left, c) (***P = 0.0014) or (left, d) (***P = 0.0031) for 72 h. g, h Immunoblot of lysates of peritoneal macrophages from WT or Arrb2−/− mice infected with HSV-1 g or VSV h for indicated times. i, j Immunoblot analysis of monomeric and dimeric IRF3 as in g or h. k Immunofluorescence microscopy of peritoneal macrophages from WT or Arrb2−/− mice infected with PBS (left), HSV-1 (middle), or VSV (right) for 8 h. l Immunoblot analysis of nuclear (Nuc) and cytoplasmic (Cyt) fractions as in i. m Ifnb mRNA levels in RAW264.7 cells transfected with control siRNA or β-arrestin 2 siRNA and then stimulated with HSV-1 (left), VSV (middle), or ISD (right) for indicated times (***P < 0.0001, < 0.0001 in sequence, left panel; ***P < 0.0001, *P = 0.0125, middle panel; *P = 0.0239, right panel). n The virus titers as in m for 72 h (***P = 0.0031, left panel; *P = 0.0019, right panel). o, p Immunoblot of lysates of RAW264.7 cells treated with indicated siRNA and infected with HSV-1 o or VSV p for indicated times. q Immunoblot analysis of monomeric and dimeric IRF3 as in o. ns not significant. Data are representative of at least three independent experiments (mean ± SEM. in b–f, m, n, n = 3). Two-tailed unpaired Student’s t-test.