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. 2020 Nov 26;11:5998. doi: 10.1038/s41467-020-19782-x

Fig. 5. Syndecan-1 loss impairs established bcCML growth in human and mouse models.

Fig. 5

a Approach to assess the impact of Sdc1 loss on established bcCML. b Colony formation of shCTRL or shSdc1 bcCML **P = 0.003, ***P = 0.0003 (n = 3 technical replicates, representative of three biological replicates). c Impact of ectopic Sdc1 expression in Lin+ bcCML. *P = 0.02 from Lin−CTRL, #P = 0.01 from Lin+CTRL (n = 3 technical replicates). d Representative chimerism of shCTRL or shSdc1 bcCML. n = 3 mice/group. *P = 0.02. e Survival curve of mice transplanted with shCTRL or shSdc1 bcCML. **P = 0.003 (n = 4 for shCTRL and n = 7 for shSdc1). f Colony formation of shCTRL or shSdc1 AML cells. ****P < 0.0001, (n = 3 technical replicates). g Impact of shSdc1 on AML. *P = 0.03 (n = 33 for shCTRL and n = 15 for shSdc1). h Impact of shSDC1 on K562. ****P < 0.0001 (n = 3 technical replicates). i Impact of shSDC1on MV4-11. Data normalized to shLacZ control. ***P = 0.001 (n = 3 technical replicates from two biological replicates). j Impact of shSDC1 on primary patient AML. *P < 0.01, **P = 0.008 (n = 3 technical replicates). Two-tailed unpaired Student’s t-tests were used to determine statistical significance. Source data are provided as a Source Data File.