Subject Area	Environmental Science
More specific subject area	Assessment of microplastics abundance in lake sediments
Protocol name	Method for microplastics extraction from lake sediments
Reagents/tools	The method did not require no any specific equipment or resources and can be reproduced in any chemical laboratory. Glassware: Petrie dishes, glass beakers, water bath, glass funnels, desiccator, squirt bottles, filter nets with mesh size 333, 174 and 100 mm, stereomicroscope Reagents H2O2, HCl, HCOOK, FeSO4·7H2O, distilled water
Experimental design	Method involves preliminary wet peroxide oxidization of sediment sample, sediment flushing to remove fine grained fractions, density separation with potassium formate, wet peroxide oxidization, HCl digestion, optional density separation, filtration, drying and microplastics analysis under the microscope.
Trial registration	n/a
Ethics	n/a
Value of the Protocol	Describe the importance of the protocol in up to 3 bullet points •Allows microplastics extraction from organic-rich sediments •Quality assurance and control procedures are provided