Fig. 6.

Myogenic differentiation analysis of C2C12 myoblasts regulated by hydrogels. (A) Immunofluorescence staining of MHC protein in C2C12 after treated with FPCP hydrogel and controls for 7 days, the NC group without hydrogel was used as a control, myotubes and nuclei were stained as green and blue, respectively (scale bar: 200 μm, n = 3); (B–E) Myotube number (B), myotube diameter (C), myotube length (D) and fusion index (E) calculated according to at least three immunofluorescence images of each sample using Image J software (*p < 0.05, **p < 0.01).