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. 2020 Nov 13;10:541262. doi: 10.3389/fonc.2020.541262

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Copyright © 2020 Li, Cao, Huang, Wu, Chen, Wang and Xiong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MTA1-knockdown restricts the autophagy process in MCF-7 cells. (1) NC_MCF-7 and shMTA1_MCF-7 cells are planted in 24-cell plates for 24h, followed by 48h-treatment of 5μM GEM or DMSO, then for IF assay for autophagy (A). (2) NC_MCF-7 and shMTA1_MCF-7 cells are planted in 6-cell plates for 24h, followed by 48h-treatment of 5μM_GEM or DMSO (4 groups: NC_DMSO, NC_GEM, shMTA1_DMSO and shMTA1_GEM), then for WB assay for autophagy and apoptosis associated protein (B, D_1-5). (5) NC_MCF-7 and shMTA1_MCF-7 cells are planted in 24-cell plates for 24h, followed by 48h-treatment of 5μM_GEM or DMSO (4 groups: NC_DMSO, NC_GEM, shMTA1_DMSO and shMTA1_GEM), then for mitochondrial viability assay (C).