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. 2020 Nov 13;14:571351. doi: 10.3389/fnana.2020.571351

FIGURE 2.

FIGURE 2

Endogenous NMDA receptors render neurons sensitive to NMDA. At DIV 10, the vast majority of (A) pyramidal cells and of (B) interneurons responded with dendritic beading to NMDA (50 μM) applied for 10 min. Preincubation with Ro25-6981 (5 μM) was neuroprotective (ANOVA on ranks versus control, *p < 0.05). TCN201 [10 μM] was unable to protect. Antagonists had been applied 60 min before the treatment with 50 μM NMDA, followed by immediate fixation. The average percentage of pyramidal neurons (in total 523 cells analyzed) and multipolar non-pyramidal neurons (in total 218 cells analyzed) with dendritic beading per culture was plotted as mean ± S.E.M. 6–10 OTC per condition were analyzed. Only completely stained neurons identifiable by axonal projections were assessed.