FIGURE 6.

Higher expression of Y1472 phosphorylated GluN2B during recovery from ifenprodil treatment. The five nitrocellulose strips used to detect six proteins of different molecular weight [in kDa] derive all from one gel. Per lane, the lysate of 1 OTC (each about 1 mg wet weight) was loaded. Shown are arbitrarily paired OTC from one batch of cultures, two OTC at 12 h and two OTC at 24 h after washout of ifenprodil, and two OTC that were mock-stimulated, and harvested at the 24 h time point. Note that the level of GluN2B Y1472 phosphorylation was higher in the recovery cultures, the GAD-65 level was lower under ifenprodil and still lower after 12 h recovery; the PSD-95 levels were not affected. Tubulin and actin served as house-keeping proteins. For quantification see Table 5.