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. 2020 Nov 13;14:571351. doi: 10.3389/fnana.2020.571351

TABLE 1.

Plasmids and NMDA receptor antagonists.

Plasmid, Promoter Source Catalog #
pEGFP-N1 in pcDNA3.0, CMV Clontech, Heidelberg, Germany cat# 632370
pmCherry-N1, pcDNA3.0, CMV Clontech, Heidelberg, Germany cat# 632523
mKate (pTagFP635), CMV Gift from Dr. Thomas Hughes n.a.
GluN1-1a-pcDNA3, CMV Gift from Michael Hollmann n.a.
GluN2A-pcDNA3, CMV Gift from Michael Hollmann n.a.
GluN2A-pEGPF, CMV Gift from Michael Hollmann n.a.
GluN2B-pcDNA3, CMV Gift from Michael Hollmann n.a.
GluN2B-pEYPF, CMV Gift from Michael Hollmann n.a.
Reagent, final concentration
Ifenprodil, 10 μM Sigma-Aldrich cat# I2892
Ro25-6981, 5 μM Sigma-Aldrich cat# R7150
NVP-AAM077, 200 nM Sigma-Aldrich cat# P1999
TCN201, 10 μM Tocris cat# 4154
CX546, 5 μM, 25 μM Sigma-Aldrich cat# C271
NMDA, 50 μM Sigma-Aldrich cat# M3262
DL-AP5, 5 μM Alomone Labs cat# D-140
L-glutamate, 500 μM and 1 mM Sigma-Aldrich cat# 49449