TABLE 5.

Ifenprodil treatment decreased GAD expression and increased phosphorylation of GluN2B.

Protein	Control	Ifenprodil	12 h recovery	24 h recovery	p
GluN2B	1.0 ± 0.06 (6)	1.05 ± 0.05 (3)	n.d.	1.04 ± 0.06 (3)	n.s.
ph-GluN2B	1.0 ± 0.04 (10)	n.d.	1.23 ± 0.052 (5)	1.26 ± 0.049 (6)	0.009
GluN1	1.0 ± 0.02 (3)	n.d.	1.04 ± 0.01 (3)	1.02 ± 0.02 (3)	n.s.
PSD-95	1.0 ± 0.038 (19)	1.09 ± 0.041 (6)	1.034 ± 0.06 (6)	0.98 ± 0.019 (11)	n.s.
GAD-67	1.0 ± 0.027 (18)	0.88 ± 0.02 (6)	0.89 ± 0.014 (7)	0.99 ± 0.016 (12)	<0.001
GAD-65	1.0 ± 0.016 (18)	0.78 ± 0.038 (6)	0.84 ± 0.015 (7)	0.92 ± 0.013 (12)	<0.001

OTC from three preparations were either treated with ifenprodil [10 μM] from DIV 6–10, and harvested at DIV 10 (ifenprodil), or washed two times with fresh medium and maintained for 12 and 24 h (recovery) without ifenprodil. Control cultures were mock-stimulated with water. For the recovery, control cultures were harvested at the 24 h time point. Gel by gel, to generate a S.E.M. also for the controls, control values were normalized to the average of each group of control values which was set to 1; thereafter, all controls were pooled. Reported are the mean ± S.E.M. The number of blots run for every protein/epitope is given in brackets. Statistical comparison was done by ANOVA on ranks versus control; in italics, p-values are given. In bold, values significantly different from control; n.s., not significant; n.d., not determined.