TABLE 6.
(A) Pyramidal cells transfected with GluN2A and GluN2B encoding plasmids from DIV 5–10 | ||||
Pyramidal cells of layers II/III |
Pyramidal cells of layers V/VI |
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Condition (no. of batches) | ADL (n) Segments | BDL Segments | ADL (n) Segments | BDL Segments |
Control | 1248 ± 52 (93) 29 ± 1.4 | 213 ± 10 5.6 ± 0.3 | 1024 ± 38 (118) 19 ± 0.7 | 261 ± 13 5.3 ± 0.2 |
GluN2A (4) | 1132 ± 46 (65) 26 ± 1.3 | 256 ± 15 6.1 ± 0.4 | 1065 ± 39 (81) 19 ± 0.8 | 286 ± 15 5.6 ± 0.3 |
GluN2B (4) | 1183 ± 47 (100) 27 ± 1.2 | 253 ± 17 6.0 ± 0.4 | 1051 ± 30 (114) 20 ± 0.7 | 265 ± 12 5.9 ± 0.3 |
ANOVA on ranks vs. control | P = 0.58 P = 0.47 | P = 0.09 P = 0.51 | P = 0.3 P = 0.21 | P = 0.33 P = 0.10 |
(B) Interneurons transfected with GluN2A and GluN2B encoding plasmids from DIV 5–10 | ||||
Condition (no. of batches) | MDL (n) | MDS | no. of PD | |
Control | 362 ± 16 (81) | 6.1 ± 0.3 | 5.1 ± 0.2 | |
GluN2A (4) | 375 ± 17 (90) | 6.2 ± 0.3 | 4.8 ± 0.2 | |
GluN2B (4) | 409 ± 21 (73) | 6.9 ± 0.3 | 5.0 ± 0.2 | |
ANOVA on ranks vs. control | P = 0.32 | P = 0.06 | P = 0.61 |
Given is the mean ± S.E.M, and, in italics, the p-values of an ANOVA on ranks. For pyramidal cells in (A): ADL, apical dendritic length [μm]; BDL, the average basal dendritic length per cell [μm]; the number of dendritic segments; for interneurons in (B): MDL, mean dendritic length [μm] per cell; MDS, mean dendritic segments; no. of PD, number of primary dendrites; n, number of neurons analyzed.