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. 2020 Nov 13;14:571351. doi: 10.3389/fnana.2020.571351

TABLE 6.

Effects of transfection with plasmids encoding GluN2A and GluN2B.

(A) Pyramidal cells transfected with GluN2A and GluN2B encoding plasmids from DIV 5–10
Pyramidal cells of layers II/III
Pyramidal cells of layers V/VI
Condition (no. of batches) ADL (n) Segments BDL Segments ADL (n) Segments BDL Segments
Control 1248 ± 52 (93) 29 ± 1.4 213 ± 10 5.6 ± 0.3 1024 ± 38 (118) 19 ± 0.7 261 ± 13 5.3 ± 0.2
GluN2A (4) 1132 ± 46 (65) 26 ± 1.3 256 ± 15 6.1 ± 0.4 1065 ± 39 (81) 19 ± 0.8 286 ± 15 5.6 ± 0.3
GluN2B (4) 1183 ± 47 (100) 27 ± 1.2 253 ± 17 6.0 ± 0.4 1051 ± 30 (114) 20 ± 0.7 265 ± 12 5.9 ± 0.3
ANOVA on ranks vs. control P = 0.58 P = 0.47 P = 0.09 P = 0.51 P = 0.3 P = 0.21 P = 0.33 P = 0.10

(B) Interneurons transfected with GluN2A and GluN2B encoding plasmids from DIV 5–10
Condition (no. of batches) MDL (n) MDS no. of PD

Control 362 ± 16 (81) 6.1 ± 0.3 5.1 ± 0.2
GluN2A (4) 375 ± 17 (90) 6.2 ± 0.3 4.8 ± 0.2
GluN2B (4) 409 ± 21 (73) 6.9 ± 0.3 5.0 ± 0.2
ANOVA on ranks vs. control P = 0.32 P = 0.06 P = 0.61

Given is the mean ± S.E.M, and, in italics, the p-values of an ANOVA on ranks. For pyramidal cells in (A): ADL, apical dendritic length [μm]; BDL, the average basal dendritic length per cell [μm]; the number of dendritic segments; for interneurons in (B): MDL, mean dendritic length [μm] per cell; MDS, mean dendritic segments; no. of PD, number of primary dendrites; n, number of neurons analyzed.