Fig. 1.
YTHDC2 expression was suppressed in LUAD and correlated with poor clinical outcome. (A–C) Expression level of m6A readers in the normal lung and LUAD tissues from GEPIA (A, B) and Oncomine (C) databases. (D) Overall survival curves based on YTHDC2 expression in LUAD were obtained from Kaplan-Meier plotter database. (E–G) YTHDC2 mRNA and protein expression in paired adjacent-tumor tissues from cohort#1, as measured by RT-qPCR, ELISA, and IB assay. (H, I) Representative IHC images of YTHDC2 in paired adjacent-tumor tissues from cohort#1 TMA, scar bar 100 μm. IHC staining was scored and analyzed, and the percentage for patients with indicated alteration of YTHDC2 is also shown. (J, K) Correlations between YTHDC2 expression and tumor diameter and between YTHDC2 expression and tumor stage. Statistical analysis was performed using Student's t tests (E, F) and Chi-squared test (J, K). Data are means ± SEMs, *p < 0.05, **p < 0.01.