Figure 3.

The loss of ChREBP in L.G6pc^−/−livers induces a severe liver phenotype. Control and L.Chrebp^−/− mice (pink bars) and L.G6pc^−/− and L.G6pc^−/−.Chrebp^−/− mice (red bars) were fed a standard diet during 10 days after tamoxifen treatment. (A) Plasma concentrations of AST and ALT transaminases (n = 6–8/group). (B) Plasma concentration of albumin (n = 6–8/group). (C) Representative pictures of HPS staining and Trichrome's Masson (TM) staining of control (WT), L.G6pc^−/−, L.Chrebp^−/− and L.G6pc^−/−.Chrebp^−/− liver sections. Bars represent 100 μm. (D–E) Relative mRNA expression of Il6 (coding for interleukin 6), Tgfa, Crp, Pai and Tgfb1 (n = 6/group); western blot analyses of MCP1 (60 μg protein), TGFβ1 and vimentin (n = 6–8/group) in the livers. Representative images of western blots (n = 3) are shown above the quantification graph (n = 6–8/group). The quantification of western blots was performed relatively to the total amount of proteins using stained-free imaging technology (see Figure S5). Data are expressed as mean ± s.e.m. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 indicate significance compared to control mice; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 indicates significance compared to L.G6pc^−/− mice; ^£p < 0.05, ^££p < 0.01 and ^£££p < 0.001 indicate significance compared to L.Chrebp^−/− mice. See also Figures S3 and S4.