Figure 4.

The loss of hepatic ChREBP in L.G6pc^−/−mice induces hepatocyte cellular stress. Control and L.Chrebp^−/− mice (pink bars) and L.G6pc^−/− and L.G6pc^−/−.Chrebp^−/− mice (red bars) were fed a standard diet during 10 days after tamoxifen treatment. Quantification of proteins involved (A) ER stress (BiP, Ire1α, ATF4 and CHOP) and (B) apoptosis (caspase 3, caspase 7 and PARP) by western blot analyses of protein extracts from the livers (n = 6–8/group). The quantification of proteins was performed relatively to the total amount of proteins using stained-free imaging technology (see Figure S5). For pro-apoptotic proteins, the cleaved protein quantity was expressed relatively to the total isoform. Representative images of western blots (n = 3) are shown above the quantification graphs (n = 6–8/group). (C) Relative mRNA expression of Fgf21in the liver (n = 4–6/group) and plasmatic FGF21 concentration (n = 6–8/group except for L.Chrebp^−/− mice where n = 3). Data are expressed as mean ± s.e.m., relatively to control mice. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 indicates significance compared to control mice; ^$p < 0.05, ^$$p < 0.01 and ^$$$p < 0.001 indicate significance compared to L.G6pc^−/− mice; ^£p < 0.05, ^££p < 0.01 and ^£££p < 0.001 indicate significance compared to L.Chrebp^−/− mice.