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. 2020 Nov 13;22:e00189. doi: 10.1016/j.plabm.2020.e00189

Table 2.

Intrarun assay.

Panel 1
Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
CA9 Low Control Mid Control
MEAN Dose (pg/mL) 15 57 246 16 51 245
Std. Deviation 0.97 3 8.1 0.67 2 6.1
% CV 6.6% 4.6% 3.3% 4.3% 4.7% 2.5%
 
IL8 Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
Low Control
Mid Control
MEAN Dose (pg/mL) 17 67 282 18 59 272
Std. Deviation 0.89 3 7.6 0.72 2 5.3
% CV 5.4% 3.8% 2.7% 4.0% 4.0% 2.0%
 
MMP9 Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
Low Control
Mid Control
MEAN Dose (pg/mL) 270 1114 4643 296 994 4601
Std. Deviation 11.99 71 103.6 10.36 37 109.2
% CV 4.4% 6.3% 2.2% 3.5% 3.7% 2.4%
 
VEGF Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
Low Control
Mid Control
MEAN Dose (pg/mL) 38 162 713 41 126 626
Std. Deviation 1.74 6 20.4 1.45 4 18.6
% CV 4.5% 3.5% 2.9% 3.6% 3.2% 3.0%
 
Panel 2
Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
ANG
Low Control
Mid Control
MEAN Dose (pg/mL) 57 226 910 55 226 911
Std. Deviation 5.30 6 21.0 1.70 5 36.3
% CV 9.3% 2.5% 2.3% 3.1% 2.3% 4.0%
 
APOE Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
Low Control
Mid Control
MEAN Dose (pg/mL) 1308 5042 17833 1021 4124 16924
Std. Deviation 131.33 152 506.0 32.30 155 589.7
% CV 10.0% 3.0% 2.8% 3.2% 3.7% 3.5%
 
A1AT Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
Low Control
Mid Control
MEAN Dose (pg/mL) 1211 4527 18254 1144 4459 18669
Std. Deviation 187.72 124 353.8 82.00 146 432.7
% CV 15.5% 2.7% 1.9% 7.2% 3.3% 2.3%
 
PAI1 Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
Low Control
Mid Control
MEAN Dose (pg/mL) 62 239 949 57 225 920
Std. Deviation 6.78 7 20.9 1.40 5 25.0
% CV 11.0% 2.7% 2.2% 2.5% 2.2% 2.7%
 
SDC1 Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
Low Control
Mid Control
MEAN Dose (pg/mL) 632 2471 9977 596 2365 9465
Std. Deviation 67.36 73 260.3 17.80 65 361.0
% CV 10.7% 2.9% 2.6% 3.0% 2.7% 3.8%
 
Panel 3
Diluent Set 1
Diluent Set 2
High Control Low Control Mid Control High Control
MMP10
Low Control
Mid Control
MEAN Dose (pg/mL) 89 363 1529 107 403 1536
Std. Deviation 4.69 18 80.9 3.40 12 29.0
% CV 5.3% 5.0% 5.3% 3.2% 2.9% 1.9%