Figure 8.

NP-NF in the peripheral retina of patients with ALS. NP-NF is shown in red using SMI 32, with nuclei counterstained in blue with DAPI. NP-NF-positive spheroids ranging from 7 to 10 µm were observed in the RNFL in the ALS group (B) but not in controls, where the NP-NF signal was confined to the RGC cell body (A). (A) Control (case 3); (B) ALS (case 5). Abbreviations: RNFL = retinal nerve fiber layer; GCL = ganglion cell layer; IPL = inner plexiform layer; INL = inner nuclear layer; OPL = outer plexiform layer. Scale bar = 50 µm.